Optimization of the effect of ultrasound and glucose added on the Saccharomyces cerevisiae PTCC 5269 biomass using response surface methodology (RSM)

The yeast Saccharomyces cerevisiae has a strong fermentation ability and very high efficiency in the fermented industry and fermented products. There are several ways to increase the growth of the yeast biomass. One of these methods is the use of ultrasound. Stimulating by low intensity ultrasound increased output of enzymes from the cell wall and membrane permeability, it can improve the speed and efficiency of handling the growth and reproduction of cells, and have appropriate applications for yeast growth and single cell protein production. In this study using the yeast Saccharomyces cerevisiae PTCC5269. Optimization of ultrasound power was (2, 6, and 10 watts) and time (10, 20, 30 sec) applied ultrasonic low intensity and low glucose concentrations (5, 10, and 15% v/v) and studied on growth performance, using response surface methodology (RSM). Optimum operating conditions obtained from RSM was the power of ultrasound 6 W, sonication time of 20 seconds and add 10% glucose. Minimum and maximum of produced biomass was 9.1 milligram and 43.3 milligram. Results showed that the dry weight increased with sonication. Maximum amount of yeast biomass obtained after applying average ultrasound power and time. The samples treated with ultrasound shows significant increase in biomass compared with the untreated samples. With increasing cell growth, the conditions being optimal for the use of the yeast Saccharomyces cerevisiae as a single-cell protein due to the probiotic properties of yeast.