The effect of ascorbic acid on reduction of oxidative stress caused by salinity in potato

In this study, the effect of exogenous ascorbic acid treatment, as a strong antioxidant, on the enzymatic and non-enzymatic antioxidant system and oxidative stress was investigated in Solanum tuberosum L. cv. Carlton in vitro. Explants were cultured in liquid MS medium containing different concentrations of ascorbic acid (0, 0.5 and 1 m M) and NaCl (0 and 75 m M) for 4 weeks. NaCl stress reduced growth parameters and photosynthetic pigments, carotenoids, ascorbate pool, phenolics content and increased lipid peroxidation, electrolyte leakage, H2O2 level, proline content and the activity of lipoxygenase (LOX), superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (GPOD), ascorbate peroxidase (APX), and glutathione reductase (GR). Ascorbic acid via increasing most enzymatic antioxidants (SOD, CAT, GPOD, APX and GR) and most non-enzymatic antioxidants and proline, reduced lipid peroxidation, electrolyte leakage, H2O2 content and LOX activity and leading to promote protective reactions which were reflected in improving plants growth parameters.