Trichostrongylus colubriformis: Possible Most Common Cause of Human Infection in Mazandaran Province, North of Iran

Infection with Trichostrongylus spp. is common among human and herbivorous in most parts of Iran, especially in southern and northern ar­eas. The aim of present study was to identify Trichostrongylus spp. among hu­man population using excreted egg specimens, by the molecular method, in Ma­zandaran Province, northern Iran. Overall, 33 positive fecal specimens were randomly sampled and ex­amined. PCR amplification of ITS2-rDNA region was performed on the iso­lated egg and then a restriction fragment length polymorphism (RFLP) pro­file was considered to discriminate of Trichostrongylus spp. A total of 33 positive fecal specimens, 29(78.9%), 4(12.1%) were found T. colubriformis and T. axei respectively. Our data appear the molecular evi­dence of both human T. colubriformis and T. axei infections in North of Iran. T. colubriformis was the probable most common zoonotic species caus­ing human trichostrongylosis infection in the area.