The survey effect of BAP and TDZ on direct shoot regeneration from node explant of Agastache foeniculum

The first successful and an efficient regeneration system on in vitro direct plant regeneration of Agastache foeniculum was reported. The experiment were done in factorial design with 3 replications and explants were cultured in MS media fortified with different concentrations of BAP and TDZ (0, 2.2, 4.4, 8.8 µM) in combination with 3 levels of IAA (0, 1.1 and 2.2 µM). ANOVA analysis showed that direct shoot induction and regeneration was significantly influenced by hormones combinations. In BAP and IAA treatments, highest frequency of shoot induction (32.72) and maximum regenerated shoots (7.9 shoots per explants) was observed in MS media containing BAP (8.8 µM) alone. Although in TDZ and IAA treatments, highest average number of induced shoots (24.6) and (7.13 shoots per explants) were obtained in MS media fortified with 2.2 µM of TDZ. In second experiments, effect of basal media and different concentrations of IAA and IBA on root induction was analyzed. Profuse rooting was obtained in 74% of plantlets on 1/2 MS media containing (1.1µM) IBA and highest mean rooting (5.06 roots per seedlings) were obtained in same media. The well rooted plantlets were acclimatized successfully in greenhouse on perlite media and transferred to soil with 100% survival.