The comparison of natural and synthetic scaffolds protective capabilities as a suitable growth medium for adipose-derived mesenchymal stem cells

Aim and Tissue engineering is a science using advanced techniques to repair damaged tissue and cause their recovery and reconstruction. The success of this process requires the presence of optimal and effective factors. Among the important factors in this success is to use appropriate scaffolds with wonderful mechanical and biological properties to provide an optimal growth environment and appropriate condition for the differentiation of stem cells. In this research, the evaluation of PRP and PLGA scaffolds efficiency in producing suitable environment for growth of mesenchymal stem cells was carried out. In this study, after preparing PRP and PLGA scaffolds and isolating mesenchymal stem cells from adipose tissue, the cells were seeded separately into two scaffolds and after 48 hours of cell seeding, the cells viability was evaluated by MTT assay. Our findings showed that both PRP and PLGA scaffolds were used as an ideal environment for the culture and proliferation of mesenchymal stem cells. The obtained results of this study using MTT assay indicated that when the active PRP are used with mesenchymal stem cells, its efficiency was higher than PLGA. It seems that using PRP-derived scaffolds as a protective scaffold is better to be considered for mesenchymal stem cells growth to provide effective strategies for the development of tissue engineering and regenerative medicine.