Comparison of fermentation time effect on phenazine 1-carboxylic acid (PCA) anti-corrosion antibacterial activity extracted from Pseudomonas aeruginosa MUT3 strain against steel corrosive bacteria

Aim and The most destructive types of corrosion are biocorrosion which about 20-40 percent of corrosion recompense are related to it. Natural phenazines in secondary metabolites of bacteria have been receiving increasing attention in recent years due to their potential usage as antibiotics. Steel corrosive bacteria isolated from marine source as the main cause of sea structure corrosion was studied to assay anticorrosion antibacterial material effects on the activity of this microorganism. In vitro antibacterial production of phenazine 1-carboxylic acid (PCA) was evaluated for seven days in batch fermentation. The experiments showed maximum mass production is related to sixth day with 0.313 g/l products, but the antibacterial activity was tested and the inhibition zone on the fourth day was bigger than the other days and analysis of high pressure liquid Chromatography (HPLC) indicates more purity of PCA on this day. According to the result, future increase in PCA production was achieved after four days fermentation. Therefore, in future prospects, we will try to formulate these compounds as anti-corrosive paints for industrial use.