Comparison of Antibody Titers against the Single, Mixed, Fused and Recombinant proteins, StxB, IpaD and StxB- IpaD
Message:
Abstract:
Introduction
IpaD protein plays an im-portant role in invasion, pathogenesis and infection caused by Shigella and E. coli. Another major virulence factor in Shigella dysenteriae type 1 and E. coli O157: H7 is Shigella entrotoxin or (StxB). A suitable candidate vaccine could be made by a mixture or fusion of IpaD and STxB pr-oteins. In this study, the immunogenicity and antibody titer of fused mixed and separated recombinant proteins, IpaD and STxB, in mice were compared with each other.
Materials and Methods
In this experimental study, recombinant vectors containing the gene sequences stxB-IpaD, IpaD and stxB, were prepared and also transferred into the E. coli BL21 DE3 bacterium. The protein expression levels were assessed and their expression was approved by using of We-stern blot technique. After purification of recombinant proteins with the column of chromatography, they were injected four times consecutively to the mice.
Findings
The antibody titers were statisti-cally different for STxB, IpaD, STxB-IpaD and IpaD mixed with STxB antigens in the mice, and antibody levels increased by adding the StxB to IpaD.Discussion &
Conclusion
The results of this study indicate that the furin linker sep-arated by semi furins and the produced pro-tein from mixture and fusion genes, ipaD and stxB, can increase STxB immunege-nicity effect, and could be a good candidate for the production of recombinant vaccines against Shigella and E. coli types.
Language:
Persian
Published:
Ilam University of Medical Science, Volume:22 Issue: 7, 2015
Pages:
87 to 95
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