In vitro evaluation of aquatic garlic (Allium sativum) extract effects on animal nematodes larvae

Herbal therapy using extract or yields plant، has been considered in most countries for many years and the use of herbal has been increased because of resistance and side effects of synthetic chemical drugs in recent years. Garlic is a effective plants for treatment of several diseases and its anti parasitic effects beside of other treatment properties has been studied by many investigators. The goal of this study is conducted to evaluate treatment effects of aquatic garlic extract on larval stage of parasitic nematodes. The several dilutions of aquatic garlic extract from 10 mg/ml to 8 µg/ml was used on Strongylus spp. larvae in vitro. The mortality rate of larvae were count until 7 days. The results were compared with levamizole from 50 µg/ml to 12. 5 µg/ml dilutions. In present study، 750 µg/ml dilution and more are effective on second and third larvae stages and the mortality rate was more than 65% on first day. The effect of 2. 5 mg/ml of garlic extract is similar to levamizole at 12. 5 µg/ml level. Regarding to good effect of garlic extract on larvae stage of nematodes، it can be considered for treatment or control of nematodes infection by using proper formulation of garlic extract or daily consuming in endemic area with parasitic nematodes infection.