The effects of intermittent training and age on blood lactate (La) Level and lactate dehydrogenase enzyme (LDH) activity in male wistar rats

Physical activity and age are the factors affecting on the lactate level and lactate dehydrogenase enzyme activity. The purpose of this study was to assess the effects of intermittent training on blood lactate level and lactate dehydrogenase enzyme activity in old and yang male Wistar rats. Forty male Wistar rats were divided into two old (aged 27 months and weight 389±31 g) and young groups (aged 3 ± 4 months and weight 224±14 g) and they were selected as a case study.Each group was assigned into the training (n = 10) and control (n=10) groups randomly.Training group performed encremental intermittent exercise on a treadmill 6 times/wk for 8wk. It consisted of 10 bouts of 4 min running interspersed by 2 min of active rest. Rats were anesthetized with a mixture of ketamine (100 mg/kg b/wt) and xylazine (10 mg/kg b/wt). To measure lactate and LDH, blood sample was obtained via cardiac puncture at rest, 24 hours after the last session exercise. For data analysis, statistical significance was assessed by one way-ANOVA and Tukey's post-hoc tests. All data are reported as means ±SD. The results showed no significant differences in blood lactate level between four groups, but LDH enzyme activity in the young training group was significantly higher than the control group (p < 0/ 05). These results indicate that lactate is likely decreased by intermittent training. It is benefit for lactate clearance after exercise. Furthrermore, it seems that the effect of training on lactate clearance is similar for both young and elderly. This potential is important advantage for muscle glycogen replenishment and may in turn favor better muscle pH regulation.