Detection of Barley yellow mosaic virus by Serological and Molecular Methods in some Provinces of Iran

In order to investigate the presence of Barley mild mosaic virus, 425 samples of barley and wheat from Razavi Khorasan, Northern Khorasan, Golestan, Yazd and Western Azarbayjan were collected in spring 2011. Plolyclonal antiserum was used in order to detect BaYMV by DAS-ELISA. The highest level of BaYMV infection was found in Azarbayjan gharbi and detect inRazavi Khorasan, Northern Khorasan and Golestan. Polymerase Chain Reaction (PCR) was carried out using specific primers for coat protein. A fragment of ~392 bp corresponding to BaMMV coat protein gene were amplified by PCR. Then one PCR product of Western Azarbayjan samples was sequenced and analyzed for additional confirmation. This is the first report of occurrence of BaMMV in aforementioned provinces of Iran.