Study the effect of methyl jasmonate and salicylic acid on ‎morphological characters internal pigments of artichoke callus

Modern biotechnology and genetic engineering using techniques and special ‎laboratory tools plays an important role in the pharmaceutical industry and medicinal ‎plant propagation. Among these techniques, it can be noted that a wide range of ‎elicitors are widely used for inducing metabolites at invitro culture. Due to the ‎pharmaceutical properties of artichoke and its history, it has been deeply noticed by ‎pharmaceutical industry and its cultivation is expanding rapidly. In present study the ‎effect of two elicitor's methyl jasmonate (MJ) and salicylic acid (SA) on the callus ‎mass growth and survival of invitro culture was investigated. Callus cultures of ‎artichoke were established by transferring seedling on solidified MS medium ‎supplemented with different concentrations of salicylic acid and methyl jasmonate. ‎Four weeks after subculture, the callus was collected and weighed. Results showed ‎that the SA supplementation reduced fresh weight of callus. Contrary to fresh weight, ‎a direct increase was observed in dray weight by increasing the level of SA. Opposite ‎to SA different concentration of MJ not only did not changed the fresh weight, but ‎also reduced the dry weight of callus. Study the pigment changes under different ‎treatments of SA and MJ showed that, contrary to cartonoid a direct reduction was ‎observed in chlorophyll content by increasing in both SA and MJ concentration. ‎Finally it can be suggested that, however both SA and MJ as most important elicitors ‎increase secondary metabolite production of callus, their availability in high ‎concentration affect the cell grow activity, callus survival and the biomass production.‎