Relationship between Laboratory Exposure to Crude Oil and Anti-oxidative Responses of Tetraclita Rufotincta Barnacles

Nowadays the destructive effects of oil pollutions that produced by different resources in waters and aquatic organisms have been defined with the intention of studying effects of crude oil in different concentration, on variations of anti-oxidant enzymes level (SOD, CAT)in Tetraclita rufotincta and the possibility of introduce these enzymes as biomarkers. Metod: Barnacles Tetraclita rufotincta were sampled in Bahrekan region. Barnacles exposed to 250ppb, 125ppb, 62.5ppb, 31.25ppb, 15.625ppb and 3ppb as a control, crude oil after 24,48,72 and 96 h. 15 barnacles removed from aquarium in average. Animals were examined for levels of Antioxidants enzymes (SOD, CAT) in their tissues. this study detected that in aquarium 1, with 3ppb concentration, after 24,48,72 and 96 h Superoxide dismutase(SOD) activities shown respectively 128/98, 30/04, 75/8, 62/0 U/ml/mg protein. In aquarium 2 and 3, with 15.625ppb and 31.25ppb concentration, after the period mentioned, superoxide dismutase(SOD) activities shown respectively 104/45, 74/95, 13/57, 10 U/ml/mg protein and 69/96, 61/56, 60/5, 86/46 U/ml/mg protein. In aquarium 4 and 5, with 62.5ppb and 125ppb concentration, after the mentioned period, Superoxide dismutase(SOD) activities shownrespectively 98/79, 193/9, 42/75, 124/77 U/ml/mg protein and 69/22, 40/08, 81/86, 59/95U/ml/mg protein and in aquarium 6, with 250ppb concentration, after the mentioned period, Superoxide dismutase(SOD) activities shown respectively 62/11, 87/1, 20/71, 93/47U/ml/mg protein. The results also detected that in aquarium 1, with 3ppb concentration, after 24,48,72 and 96 h Catalase (CAT) activities shown respectively 13/29, 15/31, 15/5, 16/25 U/ml/mg protein. In aquarium 2 and 3, with 15.625ppb and 31.25ppb concentration, after the mentioned period, Catalase (CAT) activities shown respectively 13/03, 16/74, 13/65, 13/61U/ml/mg protein and 11/46, 16/54, 15/, 13/58U/ml/mg protein. In aquarium 4 and 5, with 62.5ppb and 125ppb concentration, after the mentioned period, Catalase (CAT) activities shown respectively 18/74, 11/86, 11/91, 16/22U/ml/mg protein and 21/1, 15/8, 15/04, 39/22U/ml/mg protein and in aquarium 6, with 250ppb concentration, after the mentioned period, Catalase (CAT) activities shown respectively 15/54, 18/8, 15/81, 15/97U/ml/mg protein. There was no correlation between Superoxide dismutase (SOD) activities and different concentrations of crude oil, during the animals exposed to crude oil. It also detected that CAT enzyme is sensitive parameter than SOD and that could be useful biomarker for the evaluation of contaminated aquatic ecosystems in Tetraclita rufotincta barnacles.