Antioxidant role of vitamin C on reducing oxidative stress induced subchronic toxicity of rainbow trout (Oncorhynchus mykiss) with diazinon

Nowadays, organophosphorus pesticides such as diazinon are used in various agricultural activities and controlling insect's and pest's control. They could enter surface even underground water sources during drainage process. Diazinon can cause reactive oxidative stresses (ROS) during metabolism process in the body of aquatic organisms. The aim of this study is to investigate the antioxidant effect of vitamin C on antioxidant enzyme activities and lipid peroxidation in Oncorhynchus mykiss. Sixty Oncorhynchus mykiss fish with the average weight of 121 ± 18 g [±SD] were allocated into four treatment groups: (1) control, (2) diazinon (0.1 mg L‑1), (3) vitamin C (300 mg kg‑1 in diet) plus diazinon (0.1 mg L-1) and (4) vitamin C (1000 mg kg-1 in diet) plus diazinon (0.1 mg L-1). After a period of two and four weeks, sampling was performed on their blood serums to measure Superoxide dismutase (SOD) and catalase (CAT) enzymes, total antioxidant capacity (TAC) and malondialdehyte (MDA) index. Findings indicated that the fish in diazinon treated group (without vitamin C in diet) showed significant (p