Isolation and identification of Mycoplasma agalactiae by culture and polymerase chain reaction (PCR) from milk of sheep in Masal, Guilan
Message:
Abstract:
Aim and
Background
Contagious agalactiae (C.A) is a serious disease syndrome of sheep and goat characterized by mastitis and subsequent failure of milk production, arthritis, abortion and kerato-conjunctivitis. Mycoplasma agalactiae (M. agalactiae) is the main cause of the disease in sheep and goat. The aim of this study was isolation and identification of M. agalactiae with culture and polymerase chain reaction (PCR) assay from milk of sheep in Masal, Iran
Material And Methods
A total of 25 samples were collected from milk secretion of sheep. All samples were cultured in PPLO broth supplemented for M. agalactiae isolation. The bacteria DNAs were extracted by High Pure PCR Template Preparation kit and the PCR assay was applied for the detection of Mycoplasma genus in 270bp fragment in 16S rRNA gene and M. agalactiae in 375bp fragment in lipoprotein gene from culture as well as clinical samples.
Results
Of the 25 samples, 9 (36%) yielded one of the potentially pathogenic Mycoplasmas evaluated for using PPLO agar culture diagnostic method, and 17 (68%) yielded one of the potentially pathogenic Mycoplasmas evaluated for using Mycoplasma genus PCR as diagnostic method, and 5 (29/4%) of the samples yielded M. agalactiae by using M. agalactiae PCR as diagnostic method. Of the 25 samples, 8 samples were culture positive and PCR positive, 7 samples were culture negative and PCR negative; whereas 9 samples were culture negative and PCR positive and only one sample was culture positive and PCR negative.
Conclusion
The result showed that PCR can be used as an alternative method instead of culturing to identify M. agalactiae. M. agalactiae was detected for the first time from milk of sheep in Masal, Iran and it was not the main factor of C.A there.
Language:
Persian
Published:
New Cellular & Molecular Biotechnology Journal, Volume:5 Issue: 18, 2015
Pages:
49 to 54
magiran.com/p1407782  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 990,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
دسترسی سراسری کاربران دانشگاه پیام نور!
اعضای هیئت علمی و دانشجویان دانشگاه پیام نور در سراسر کشور، در صورت ثبت نام با ایمیل دانشگاهی، تا پایان فروردین ماه 1403 به مقالات سایت دسترسی خواهند داشت!
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 50 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!