Determination of suitable medium for non symbiotic germination of self pollinated seeds of phalaenopsis  Kyoto'

In this study، in vitro non-symbiotic germination of self pollinated seeds، protocorms survival percentage and protocorms growth of Phalaenopsis ‘Kyoto’ were investigated. For this purpose، five different modified media include Vasin and Went (VW)، Murashing and Skoog (MS)، ½ MS، Knudson C (KC)، FAST that supplemented with two g/l peptone، 15 percent sterile coconut water or two g/l peptone together with 15 percent coconut water were used. The highest seed germination of (65. 9 percent) was observed by 30days after culture on KC with two g/l peptone and 15 percent CW. The highest protocorm survival (94. 7 percent) was observed by 50 days after culture on KC with two g/l peptone. The highest growth of protocorm (3/3 mm) was obtained by 50 days after culture on FAST containing two g/l peptone and 15 percent CW.