The Evaluating of the Toxicity of Reactive Red 120 Dye by Daphnia Magna Bioassay

Increased use of nanoparticles in industries leads to entering hazardous substances to environment. Nanoparticle toxicity due to release of toxic substances into the environment is a concern for communities. One of the common nanoparticles ingredients are zinc oxide. In this study toxicity of the solution containing the Reactive Red 120 dye after Nano-catalytic process UV/ ZnO using biological test Daphnia magna was studied. this is a fundamental – practical study، which done on laboratory scale. Toxicity assay tests were carried out using Daphnia magna a bio-indicator. Then results of toxicity tests using SPSS software were analyzed and Lc50were determined. Results showed that the Lc50 value ​​at 24،48،72،96 hr is 73. 16، 55. 93، 41. 32، 30. 45 mg/l and the toxicity unit values are 1. 36، 1. 78، 2. 42، and 3. 28، respectively. the results generally indicated that toxicity increased in process and over time and showed that Reactive Red 120 after UV/ ZnO process was toxic to Daphnia magna.