Inoculation Effects of Endophytic Fungus Piriformospora Indica and Pseudomonas Putida Bacteria on Growth and Nutrient Uptake of Wheat Plants Under Zinc Deficiency Condition

Zinc deficiency is the most widespread micronutrient disorder in the production of wheat (Triticum aestivum L.) and other cereal crops. An experiment was conducted in greenhouse, in 2013, using the sterile sand-perlite (2:1 v/v), to study the effects of two beneficial microorganisms on growth and nutritional status of wheat (Nicknejad cultivar). The study was arranged as factorial in a completely randomized design with three replications. The experimental factors consisted of Piriformospora indica (E0: Uninoculated; E1: Inoculated), Pseudomonas putida (E0: Uninoculated; E1: Inoculated) and Zinc (Zn0: 0; Zn1: 2µM ZnSO4). The results showed that inoculation by P. putida increased shoot dry weight at both levels of zinc, but this increase was observed for root dry weight only without zinc application. The iron concentration of shoot was decreased as a result of inoculation by P. putida at both levels of zinc. However, P. indica inoculation increased iron concentration in zinc application, but had no significant effect without zinc application. At both levels of zinc, the highest P, Zn, chlorophyll a and b concentrations were achieved by inoculation with P. indica. Inoculation by P. putida reduced P concentration at both levels of zinc but it reduced Zn, chlorophyll a and b concentrations only with zinc application. The results of this research showed that despite negative effect of P. putida on nutrient uptake, inoculation by P. putida and/or P. indica plays an important role in the promotion of wheat growth in zinc deficiency conditions.