Evaluation of MDM2 and P53 Expression in Dentigerous Cyst and Odontogenic Keratocyst by Immunuhistochemistry

Odontogenic keratocyst (OKC) is a developmental odontogenic cyst that has high reccurrence rate and aggressive behavior. Dentigerous cyst is the most common developmental odontogenic cyst that may transfer to neoplasm. P53 and Murine double minute2 (MDM2) are over expressed in some odontogenic cysts and tumors. The aim of this study was to compare the expression of MDM2 and P53 in OKC and dentigerous cyst and their relation to proliferative activity of these cystsby immunohistochemistry. In this cross sectional study expression of MDM2 and P53 proteins were determined in 15 cases of OKCs and 15 cases of dentigerous cysts. Results were analysed with statistical tests Chi-square, t-student and the relative significance value of 0.05 considered. Expression of MDM2 and P53 had been observed in all the odontogenic keratocysts and dentigerous cysts. Expression of both markers were higher in OKC than dentigerous and expression were higher in suprabasal layers than basal layers (P=0.045). The severity of P53 and MDM2 were high in both OKC and dentigerous cysts, and there was a statistically significant difference between MDM2 and P53 in dentigerous cysts (P=0.032), but in OKC there was no significant difference (P=0.83). Expression of MDM2 and P53 had been higher in odontogenic keratocysts than denigerous cysts. Over expression of P53 and MDM2 may be associated with pathogenesis of OKC and dentigerous cyst and may contribute to biological behavior of these cysts.