Evaluation of Antibacterial Effect of Curcumin Loaded Chitosan Nanoparticles

Nanomedicine delivery systems are known as potent therapeutictools. In addition to possessing an effective agent of turmeric، Curcumin shows antibacterial properties as well. Curcumin is not water-soluble and it can be solved in water via nanotechnology-base methods. Chitosan is a natural and biodegradable substance that is utilized for the Production of Nanoparticles (NPs) carrying drug. In the following investigation، curcumin is loaded in chitosan NPs and ultimately، the resulting nano-drug is studied as an antibacterial agent. In this study، NPs are produced using chitosan and Tripolyphosphate (TPP) salt. Curcumin solution was loaded in chitosan NPs during their production. Next، the skins of BALB/c mice infected with staphylococcus aureus are treated by curcumin-loaded chitosan NPs for 3 days. Afterwards، in order to evaluate the antibacterial property of the nano-drug، these skin suspensions of mice are cultured in bacterial medium. Dynamic Light Scattering (DLS) reveals the charge of + 7 ± 2 mV and the size of 160 ± 10 nm in curcumin-loaded chitosan NPs. Moreover، Transmission electron microscopy (TEM) and atomic force microscopy (AFM) indicates a spiral shape. Therefore، the evaluation of the optical density by spectrophotometry demonstrates that 75 ± 2 % of curcumin are loaded in chitosan NPs. Bacterial culture shows that curcumin-loaded chitosan NPs significantly inhibited staphylococcus aureus growth. This study demonstrates that curcumin-loaded chitosan NPs can be applied as a potent agent in treatment of bacterial skin infections.