Evaluation of the Antibacterial Effect of Methanol and Aqueous Extracts of Vaccinium Arctostaphylos Fruit against Salmonella spp in vitro

Salmonella infections، or salmonellosis such as typhoid، bacteremia، enterocolitis are the major health problem worldwide، especially in developing countries including Iran. This study aimed to evaluate the antibacterial effect of methanol and aqueous extracts of Vaccinium arctostaphylos fruit against Salmonella spp. The present study is an experimental one Methanol and aqueous extracts of Vaccinium arctostaphylos fruit were prepared by maceration and Soxhlet method. Antimicrobial effects of the extracts were evaluated by agar diffusion method against Salmonella typhi (PTCC: 1609) and six clinical strains of Salmonella. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were determined by micro-dilution method. All of the studied strains were sensitive to the aqueous and methanol extracts of Vaccinium arctostaphylos fruit. The mean zones of inhibition were obtained in ranging from 6. 6 to 26. 6 mm. Statistical analysis showed a significant difference between the mean diameters of inhibition zone and increased extracts concentrations (p<0. 001). The MIC values were ranged from 50-200 mg/ml، whereas the MBC values from 100- 400 mg/ml. Methanol and aqueous extracts of Vaccinium arctostaphylos fruit had inhibitory activity against Salmonella spp.