prevalence of Gram Negative Bacilli Isolated from the Environment and Surfaces of hospital wards in Gorgan

Nosocomial infection is one of the main factors of mortality in hospitals. One of the most important measures to control infection in hospitals is sampling of equipment and culturing the samples. This study aimed to investigate the prevalence of gram-negative bacilli obtained from the equipment of the hospital wards. This Descriptive study was carried out on 488 specimens taken from medical and non-medical devices and equipment of various wards, via census sampling. the samples taken by sterile swabs in Tryptic Soy Broth (TSB) were inoculated in blood agar and Eosin-Methylene Blue agar, and by biochemical standard tests the isolated colonies were identified. Of 488 isolates, 157 (32.17%) were positive for the presence of gram-negative bacilli. The highest bacterial contamination was related to Enterobacter (37.58%). Of hospital wards, the highest infection related to the surgical wards (19.75%), and the highest contamination is related to laryngoscope blades (12.74%) and ECG sensor device and the monitoring interface (7%). Microbial infection with gram-negative bacilli is an overwhelming condition in hospital equipment.