Antibacterial Effects of the Essential Oils and Ethanol Extracts of the Native Plants; Ziziphora Clinopodioides on 3 Species of Urinary Tract Isolated Bacteria in Rat's Experimental Model

After Staphylococcus aureus and E.coli, Pseudomonas aeroginosa has been the third most common cause of nosocomial infections and their active opportunistic transport, occurs due to the disregarding public health, So this study was done in order to investigate the antibacterial effects of Essential oils and Ethanol extracts of the Native Plants, Ziziphora Clinopodioides on the isolated bacteria from the urinary tract of laboratory rats, as an experimental model. Under sterile conditions, required amount of urine collection was taken from 40 healthy Wistar rats and after identifying the isolates with standard microbiologic methods, to determine the antimicrobial effects of essential oils and ethanol extracts of that native plants on the isolates, MIC, MBC and antibiogram tests was performed. The results showed that both essential oils and ethanol extracts of Ziziphora Clinopodioides have inhibitory and bactericidal effects on all isolated bacteria except for Pseudomonas aeruginosa and comparison of the inhibitory and bactericidal effects of these compounds revealed that the essential oils of this plant compared to its extracts is able to inhibit the growth isolated bacteria with low concentrations. Also, there was a significant difference between the antibacterial activity of these plants (P<0.05). It seems that we can use the compounds of Ziziphora Clinopodioides as the appropriate antibacterial materials against bacteria such as opportunistic pathogens (except for Pseudomonas aeruginosa), that may cause the human infections