Bioassay of Environmental Exposure of Children to Polycyclic Aromatic Hydrocarbons Using 1-Hydroxypyrene

Polycyclic aromatic hydrocarbons (PAHs) are a group of main air pollutants which are produced by motor vehicles and are known as carcinogens.. This study was conducted to investigate the environmental exposure of children to PAHs using urinary 1-hydroxypyrene (1-OHP) measurement..Patients and A total of 260 participants enrolled in the study, aged between 6 - 12 years. Environmental exposure due to urban traffic and tobacco smoke was investigated. Morning and evening urine samples were collected and 1-OHP concentrations were measured. High-performance liquid chromatography (HPLC) with fluorescence detector, used for sample analysis, and 1-OHP concentrations in urine samples, corrected by urinary creatinine, were determined for PAHs exposure assessment. Information on environmental tobacco smoke (ETS) exposure at home and air pollution of the residential area as a measure of heavy traffic and living close to busy highways were collected using a self-administrated questionnaire.. In general, the results showed that males had higher 1-OHP levels than females (P < 0.05). The total morning levels were 0.47 ± 0.12 µmol/mol crea vs. 0.63 ± 0.15 µmol/mol crea for evening. In the presence of ETS, higher 1-OHP concentrations were observed in morning samples. In addition, living in a polluted area is strongly associated with higher levels of 1-OHP.. Taking into account that Iran is a developing country with a young population and numerous PAHs sources, environmental exposure to PAHs at these levels can be dangerous for children’s health. Furthermore, PAHs can be declared as national concerning environmental pollutants..