The effect of carvacrol on the growth inhibition and genomic destruction in prostatic cancer cells using comet assay technique

Nowadays, cancers are one of the biggest concerns of human societies. Polyphenolic compounds and antioxidants are a key factor in the prevention or treatment of various cancers. This study was aimed to investigate the effects of carvacrol as a strength antioxidant on growth inhibition and genomic destruction of prostatic cancer cells line PC3 using comet assay technique. In this laboratory-experimental study, prostatic cancer cells line PC3 with different concentrations of carvacrol were selected and the percent of cells viability was measured using MTT assay on cells line PC3. Then, it was computed inhabitation concentration of 50 percent for the growth of IC50 cells. In the next step, alkaline electrophoresis regarding to the IC50 was done for 130, 230, and 360 µM concentrations of carvacrol and 100 comet pictures were analyzed with CASP software. According to probit model, the IC50 of carvacrol for PC3 cells was 360 µM. The rate of tail to head in alkaline electrophoresis at 130, 230, and 360 µM of carvacrol concentrations was 15.9±2.1, 38.7±4.2, and 65.3±2.0 percent, respectively (P<0.05). Carvacrol as one compound of the effective polyphenoly in treatment of cancer can potentially destroy genomic cells line PC3 derived from in prostatic cancer. The genomic destruction of carvacrol on cells line PC3 is more effective in concentrations more near to the IC50.