Immobilization of Rhizopus oryzae on loofa sponge affects interacellularly produced lipases
Message:
Abstract:
Aim and
Background
lipase is a carboxylic ester hydrolase enzyme (EC.3.1.1.3), which acts on triglycerides to release fatty acids, monoglycerides, diglycerides and glycerol. With respect to the biological process advantages against chemical process plus the ability of lipase to catalyze the revers reaction (i.e., esterification) a vast chapter on this enzyme scientific applications was started. The focusing point in this study was to determine the optimum cultivation time for free and immobilized form of Rhizopus oryzae with regard to producing membrane-bound lipase. With the usage of n-butanol and oleic acid in a non-polar environment (n-hexane as solvent) the esterification reaction was carried out while the effect of molecular sieves on the final yield was considered.
Materials And Methods
Rhizopus oryzae cells in the form of immobilized on cellulose support particles or free cells are used as a whole cell bicatalyst. Colorimetric method was used for measuring the esterification activity (i.e., determination of residual free fatty acid in reaction media).
Results
Membrane-bound lipasees which are produced by both, immobilized and free form of the cell reach their maximum activity after 48 hour. Also the biocatalyst esterification activity was improved approximately 4-folds by the immobilization of fungal cell on loofa sponge. More than 96% efficiency was gained in synthesis of 1-butyl-oleate ester in the presence of 4 g molecular sieve.
Conclusion
Immobilization could improve the membrane-bound lipase esterification activity. The lipase secretion to the culture media significantly affected by cultivation time. To reach a complete esterification reaction (approximately 100%), water adsorption with use of molecular sieve is an appropriate method.
Language:
Persian
Published:
New Cellular & Molecular Biotechnology Journal, Volume:5 Issue: 19, 2015
Pages:
24 to 31
magiran.com/p1436156  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 990,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
دسترسی سراسری کاربران دانشگاه پیام نور!
اعضای هیئت علمی و دانشجویان دانشگاه پیام نور در سراسر کشور، در صورت ثبت نام با ایمیل دانشگاهی، تا پایان فروردین ماه 1403 به مقالات سایت دسترسی خواهند داشت!
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 50 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!