Effect of Gamma Radiation on pattern of protein and somaclonal variation in plant and callus of Artemisia aucheri Boiss

The aim of this study was the evaluation of possible genetic and protein pattern changes of callus and of Artemisia plant.

In this study, 2 weeks old shoot buds and shoots grown in MS medium were exposed to gamma radiation with 50,100 and 200 Gy. Then five RAPD_PCR primers, five ISSR-PCR primers for evaluation of somaclonal variation and SDS-PAGE for electrophoresis pattern of proteins in callus of Artemisia plants were studies.

Results showed that the evaluation of DNA bands amplified in the callus and plant samples treated with gamma ray compared to the untreated samples using OPA20 in plant and FPK105 and (GATA)4 in callus in PCR products was obvious but no difference was observed using other primers. In electrophoresis of protein of plant increasing of protein bands due to application of 50 and 200 Gy were observed.

Due to the possible effects by free radicals and reactive oxygen species produced by gamma radiation caused changes in genetic and gene expression.