Prevalence of mecA gene of methicillin resistant Staphylococcus spp. isolated from nosocomial infections and environmental specimens in Sanandaj Hospitals, Kurdistan, Iran
Message:
Abstract:
Background
Methicillin resistant Staphylococcus aureus (MRSA) is one of the major agents for an increasing number of serious hospital and community acquired infections. The aim of this study was to investigate on occurrence of the MRSA and mecA gene among nosocomial and environmental specimens in Kurdistan hospitals and also to determine antibiotic resistance of the isolates. Patients and
Materials And Methods
Totally 264 clinical and environmental Staphylococcus were isolated from February 2011 to June 2012 in Kurdistan medical University Hospitals, Iran, and their susceptibility patterns to different antibiotics were performed. Furthermore, agar screen method was used to determine oxacillin resistant isolates. Finally, using PCR, the oxacillin resistant isolates were tested for the presence of mecA gene.
Results
In this study, 82 of the 88 (93.18%) Staphylococcus aureus isolates were resistant to oxacillin using agar screen method. However, mecA gene was detected in 66 of the 88 strains (75%). Our results showed that the agar screen method is more reliable in determination of MRSA strains comparing with PCR.
Conclusion
The results showed that the studied MRSA were found to be with high prevalence and mecA is widespread in S. aureus isolates in Sanandaj.
Language:
English
Published:
Research in Molecular Medicine, Volume:3 Issue: 3, Aug 2015
Page:
4
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