Change the polarity of probiotic bacteria membrane in vitro

Most of probiotic Lactobacilli adhere to intestinal surfaces, a phenomenon influenced by free fatty acids. this project to increase hydrophobicity properties of bacterial cell surface and increase performance of them, was designed and for this purpose, MRS broth media including saturated (palmitic acid,stearic acid),unsaturated fatty acids(α-linolenic acid,ɣ-linolenic acid,linoleic acid, Arachidonic acid,oleic acid and….), mix of them and control(without fatty acids),for growth lactobacillus rhamnosus and a commercial starter was investigated. Extraction, saponification and methylaton of Fat-cell bacteria by gas chromatography was measured.Changes in hydrophobic and hydrophilic properties of cultured bacteria were estimated with ethylacetat(polar solvent), octan(non polar solvent) and spectrophotometric method. The results of this analysis indicated that Addition of free fatty acids to culture medium Cause changes in cellular fatty acid bacteria and the results of membrane polarity index showed that medium included mix of saturated and unsaturated fatty acids In particular in starter bacteria, have more affinity for polar solvent and starter bacteria in medium including unsaturated fatty acids causes significant increase ratio of unsaturated fatty acids to saturated in cell bacteria. Lactobacillus rhamnosus in all of experiments showed Nonpolar properties(tendency to bind to the intestinal mucosa).