Evaluation of phenolic compounds and the antibacterial effect of various extracts of aerial parts of scutellaria pinnatifida on typical food-born pathogens
Background and Objectives
Nowadays, the use of extracts of plants for cure of bacterial infections is beloved on scientists. In this study, the effect of various extracts of aerial parts of Scutellaria pinnatifida on typical food-borne pathogens was studied. Scutellaria pinnatifida from labiatae family and scutellaria genus is endemic to Khorasan province.
Material And Methods
After preparation of methanol, dichloromethane and ethyl acetate extracts with maceration method, the presence and amount of phenolic compounds was evaluated and antimicrobial activity of Scutellaria pinnatifida was examined with disc diffusion and well diffusion methods in the positive and negative bacteria.
staphylococcus aureus with highest diameter of the inhibition zone, was sensitive bacteria in disc diffusion and well diffusion methods. There wasn’t any effect on the negative bacteria. Methanol extract had highest amount of phenolic compounds. The results showed in the aerial parts of plant, ethylacetate extract had highest effect and dichloromethane extract had lowest effect on the bacteria.
The results showed that diameter of the inhibition zone of aerial parts extract of this plant on S. aureus and B. cereus were differentiable to positive control such as Gentamycin and this plant was similar to Gentamycin for inhibition of positive bacteria.
Journal of North Khorasan University of Medical Sciences, Volume:7 Issue:2, 2015
393 - 403
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