Substrate stiffness effect on mechanical behavior of mesenchymal stem cells during differentiation to smooth muscle cells
Author(s):
Abstract:
Introduction
Human mesenchymal stem cells (hMSCs) have shown a great potential in the field of regenerative medicine as well as vascular tissue engineering. Mechanical properties of the cell substrate provides an environment that influences physiological activity of cells such as proliferation, differentiation and cell cytoskeleton developments and consequently modulates functionality of in vitro engineered cells. Materials And Methods
hMSCs were cultured under two groups of control and growth factor-induced differentiation within two types of substrates with different stiffness. We used Micropipette aspiration technique to evaluate mechanical behavior of cultured cells (effective Young’s modulus (E) and creep compliance value) during their differentiation into smooth muscle cells phenotype. Results
hMSC with growth factor-induced differentiation showed significant decrease in E value in relation to both substrates in compare to control group, within 2 days of cell culture, whereas the creep compliance of these cells sharply increased. Consequently, by increasing the cultivation time to 4 to 6 days, the E values of induced-differentiation cells significantly increased in compare to control samples, with a decrease in the creep compliance of these cells. Furthermore, the state of increase or decrease in creep compliance was depended on substrate stiffness in which the cells were seeded on. Cells cultured on soft substrates showed high tendency for preserving their viscoelastic properties.Discussion
Our results provides groundwork for stem cell-based tissue engineering in order to optimize culture conditions due to effective usage of external physical cues as well as substrate properties as the regulatory mechanisms of differentiation to functional target cells.Keywords:
Language:
Persian
Published:
Koomesh, Volume:17 Issue: 1, 2015
Pages:
187 to 195
magiran.com/p1449746
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یکساله به مبلغ 1,390,000ريال میتوانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
- پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانههای چاپی و دیجیتال را به کاربر نمیدهد.
دسترسی سراسری کاربران دانشگاه پیام نور!
اعضای هیئت علمی و دانشجویان دانشگاه پیام نور در سراسر کشور، در صورت ثبت نام با ایمیل دانشگاهی، تا پایان فروردین ماه 1403 به مقالات سایت دسترسی خواهند داشت!
In order to view content subscription is required
Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!