The effect of tempreture on antioxidant enzymes activity and morphological aspects of Astragalus fridae Rech. F. in the north-west of Semnan

Astragalus fridae of family Fabaceae has farmaceutical properties، Food and dustarial value. This plant is native to the province Semnan. Aftar village located in the North West of Semnan. Its main habitat is Aftr village located northwest of Semnan. This plant is endangered of extinction. The purposes of this project included: The study of anatomical structures of aerial organs، soluble Carbohydrate content and the activity of antioxidant enzymes of plant leaves in two different times (Spring and Summer 1392). In order to survey of effect of temperature on total protein content and the activity of antioxidan enzymes (catalase، peroxidase، ascorbat peroxidase and superoxid dismutase) and soluble carbohydrate، leaves were collected in May and August. Results indicated that protein level and Soluble carbohydrate in may were less than in August، but different between means weren’t meaningful. The activity of enzymes increased in August. Results indicated that with increasing the temprature، the activity of them increased. Histological studies were stablished by using double stained of methyl green - Alumal Carmen. Petiole and leaf anatomical studies indicated that area of schloranshim in tissue samples of August were more than May which showed the plant adaptation to high temperature stress.