Determination of the effects of carbohydrates in the cell walls of wheat bran and wheat containing diets with and without enzyme on intestinal and blood enzyme activity, VFA, viscosity, bacteria and PH

A total number of 750 one day old Ross-308 broiler chickens were allocated randomly to 5 treatments with 5 replicates using a CRD statistical design. An experiment was conducted to evaluate the effects of diets content of NSP wheat and wheat bran sources with and without exogenous enzymes on gut microbial population and physiological characteristics of broilers. Treatments were control, wheat, wheat bran, wheat+ enzyme, and wheat bran+ enzyme. Effects of different treatments and supplemented enzymes groups on the intestinal microbial population were significant. The least population of gram negative, coliforms and clostridiums and the highest population of lactic acid and bifidobacteria were existed in control and enzyme supplemented groups.Maximum digesta pH was belonged to wheat and wheat bran groups. The highest and least digesta viscosity was observed in wheat bran and control treatments respectively. Control had the least and highest pancreatic amylase and lipase enzyme activity respectively. Using of wheat diets decreased the villus length but increased the cript depth. However, supplementation of those diets with exogenous enzymes restored the situation.The highest and least villus length at deudenum, jejenum and ilium were belonged to wheat+ enzyme wheat bran groups respectively. In conclusion, using of wheat and wheat bran in broiler had negative effects on productive and physiological characteristics but supplementation of its with exogenous enzymes restored the situation.