Study on the presence of yellow and stem rust resistance genes in doubled haploid lines of bread wheat using molecular markers

In this research, 150 wheat doubled haploid lines were produced using chromosome elimination method by crossing between wheat and maize. Resistance of doubled haploid lines, their parents and check cultivars against strip and stem rust was evaluated at seedling and adult plant stages. Accordingly, eight known molecular markers which are tightly linked to resistance genes including Yr5, Sr31/Yr9/Lr26, Yr15, Sr38/Yr17/Lr37, Lr34/Yr18/Pm38, Yr27, Yr36 and Yr48 were screened in parents. Results showed that molecular markers for Yr5, Yr15, Yr27 and Yr36 couldnt detect polymorphism between parents as well as positive and negative controls. For gene block Sr38/Yr17/Lr37 and locus Yr48 allele sizes were not similar to those which were expected for these genes. Results also showed that MV17 and Flanders have gene block of Sr31/Yr9/Lr26, and only 3 lines of population DH-26: Ghods*3/MV17 had this gene block from which two doubled haploid lines showed resistance reaction to TTSTK and TTKSK of Puccinia graminis pers. f.sp tritici races. Genetic test for the presence or absence of gene block Lr34/Yr18/Pm38 on parents of doubled haploid lines showed that MV17 comprises this gene block. Evaluation of doubled haploid lines for this gene block showed that 6 doubled haploid lines of population DH-26:Ghods*3/MV17 have gene block Lr34/Yr18/Pm38 for which only one doubled haploid line showed resistance reaction to Puccinia striiformis Westend f.sp. tritici race of 7E158A+, Yr27 at both seedling and adult plant stages.