Investigation of the Probiotic effect of Iranian Native Lactobacillus paracasei against Toxicity Induced by Aflatoxin B1 in vivo

Aflatoxins are known as the most important toxins which their consumption could cause acute poisoning and create carcinogenic effects. Moreover, previous studies demonstrated the ability of lactic acid bacteria to connect to aflatoxin in food material. The aim of this study is to investigate the effect of the native probiotic Lactobacillus para casei strains TD3 against toxicity induced by aflatoxin B1 in vivo. 24 wistar male rats (250±10 g) were divided into 3 groups including: one negative control group and two groups treated with aflatoxin (170 µg/kg) and Lactobacillus para casei strain TD3 isolated from Tarkhine with aflatoxin (109 cfu/day) for 4 weeks. At the end of the experiment, the blood and tissue samples were collected for histopathological and biochemical studies. The results indicated that treatment with Aflatoxin leads to a significant increase in the amount of liver enzymes such as AST, ALP and also liver damages. Furthermore, the group that received Lactobacillus para casei strain TD3, the level of these enzymes was reduced and liver damages due to aflatoxin were improved. The present study showed that aflatoxin can lead to liver damages and native Lactobacillus para casei strain TD3 which isolated from Tarkhine, probably leads to protective effects by binding to aflatoxin. Thus, it is considered as a biologic agent to remove aflatoxin in vivo.