Study of vac A genotypes of H.pylori isolated from patients with the upper gastrointestinal diseases by PCR

Helicobacter pylori is a gram negative curved bacilli which is colonized in the human stomach. It causes duodental ulcer, gastritis and is associated with adenocarinoma. There might be a possible relationship between cagA, vacA and ice a genes and clinical outcomes. The aim of this study was to identify the frequency od vacA genotypes of H.pylori isolated from the upper gastrointestinal. In tis study, 100 H. pylori strains were isolated from patients with different gastrointestinal disease in Azahra hospital. Vac A alleles were typed using PCR with specific primers. There were four Vac A mosaicsms, including 28 for s1a/ m1 (28%), 23 for s1b/m1 (23%), 26 for s1a/m2 (26%) and 23 for s1b/m2 (23%), s2 form was not found.The results showed there is no significant relationship between fifferent genotypes of vacA and the related diseases.