Cell Viability and Neural Markers Expression of Human Adipose-Derived Stem Cells after Neural Induction in Alginate Tridimensional Cell Culture
Message:
Abstract:
Background

Different studies have been done to obtain sufficient number of neuronal cells for treatment of central nervous system injuries. Adipose-derived stem cells have higher proliferation rate and more resistant to apoptosis. These cells can be induced to differentiate along several multilineage cells such as neuron-like cells. Alginate hydrogel is a polysaccharide polymer that has proper properties including biocompatibility with no immunogenicity. In this study, the effect of alginate hydrogel on the cell viability, proliferation rate and neurogenic differentiation of human adipose derived stem cells was evaluated at 14 days after induction.

Methods

Adipose-derived stem cells isolated from human, were cultured in neural induction media and seeded into alginate hydrogel. The cell viability and differentiation efficiency of encapsulated cells were evaluated via doubling time and real time quantitative reverse transcription polymerase chain reaction (real time RT-PCR) analysis, respectively. The collected data was analyzed using one-way ANOVA test.

Findings

The rate of proliferation of encapsulated cells significantly decreased as compared with control group (P < 0.001). The expression of nestin, glial fibrillary acidic protein (GFAP) and microtubule-associated protein 2 (MAP2) markers significantly decreased as compared with control group (P < 0.001).

Conclusion

Altogether cell viability and proliferation rate of encapsulated cells in alginate hydrogel significantly decreased as compared with control group, but alginate hydrogel can promote neural differentiation of adipose-derived stem cells.

Language:
Persian
Published:
Journal Of Isfahan Medical School, Volume:33 Issue: 350, 2015
Page:
1
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