An Evaluation of Antibacterial activity of Ethanolic Extract of sour tea (Hibiscus Sabdariffa) against Klebsiella Pneumoniae Resistant to antibiotics

Background and The present study was carried out to determine the potential antibacterial agent of ethanolic extract of Hibiscus Sabdariffa against Klebsiella Pneumonia isolation of urinary tract infections. Methods and Materials:The leaf H. Sabdariffa was properly dried and pulverized into a coarse powder as described by Hanafy. Twenty grams of grinded powders from each plant were soaked in 60 ml of ethanol (95 %v/v) plus water, separately for one day with occasionally shaking. After one day of dissolving, materials were filtered through a Whatman no. 1 filter paper. Then the filtrates were evaporated using rotary evaporator. At last, 0.97 g of dried extract was obtained and then stored at40C in an air tight screw-cap tube. Isolation of bacteria: All 12 K. Pneumoniae were isolated from urine culture of hospitalized patients (Amir Al-Momenin Hospital, Zabol, south-eastern Iran), whom suffered from urinary tract infections during the years 2010- 2011. Isolated bacteria were identified by Gram's stain and standard biochemical tests. Sensitive strains to several antibiotics were evaluated by the disk diffusion method with standard Kirby - Bauer. Results showed Klebsiella Pneumoniae isolates resistant to the antibiotics Erythromycin (75%), Cefixime (3/58%) and Ceftazidime (3/33%). The results showed that the ethanol extract of Hibiscus tea with 10 ml / mg and l5 mg/m density have maximum inhibitory against Klebsiella Pneumonia samples and only one strain of K. Pneumoniae is inhibited in concentration 1/25. It seems that H.Sabdariffa extracts could inhibit the growth of all of the mentioned bacteria. We noticed that the bactericidal effect of H.Sabdariffa extract was less than its bacteriostatic effects.