The biological properties evaluation of the radiolabeled complex: 99mTc -DTPA- Herceptin in cancerous cell lines

Message:
Abstract:
Aim and
Background
Herceptin as a humanized monoclonal antibody against HER2 is used for the treatment of the early stage of HER2 over-expresed breast cancers. This antibody has the potential to be used as radioimmuno-scintigraphy agent in diagnosing HER2 breast cancer. In this study, the radiolabeling of Herceptin with 99mTc was evaluated for the first step in producing a new radiopharmaceutical agent.
Materials And Methods
Herceptin antibody was labeled with Technetium-99m using Diethylene Triamine Pentaacetic Acid (DTPA) as chelator. All the quality control tests were performed. Labeling efficiency, stability in buffer and serum, immunoreactivity, toxicity and cell binding of the complex were tested on different breast cancer cell lines.
Results
The labeling efficiency of the complex was 97 ± 2%. The stabilities in phosphate buffer and in human blood serum within 24 h post preparation were 96 ± 1% and 90 ± 2%, respectively. The immunoreactivity of the complex was 83 ± 3%. The binding of labeled antibody to the surface of SKBR3, MCF7 and A431 cells were increased by increasing Her2 concentration on the cells surface. No cytotoxicity effect of complex was seen on the cell lines.
Conclusion
The findings showed that 99mTc-DTPA-Herceptin can be a promising candidate as HER2 antigen scanning for human breast cancer that needs more investigations in future.
Language:
Persian
Published:
New Cellular & Molecular Biotechnology Journal, Volume:5 Issue: 20, 2015
Pages:
97 to 104
magiran.com/p1466046  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 1,390,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
دسترسی سراسری کاربران دانشگاه پیام نور!
اعضای هیئت علمی و دانشجویان دانشگاه پیام نور در سراسر کشور، در صورت ثبت نام با ایمیل دانشگاهی، تا پایان فروردین ماه 1403 به مقالات سایت دسترسی خواهند داشت!
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!