Effects of silver nanoparticles on salinity tolerance in basil plant (Ocimum basilicum L.) during germination in vitro

Aim& Basil (Ocimum basilicum) belongs to the family Lamiaceae. It is annual plant known as an important medicinal plant. To study the effects of nano-silver particles on salinity tolerance behaviors (Ocimum basilicum L.), the present research was conducted. experiments were carried out factorially based on CRD with four replicates during autumn 2013. Samples were treated with 6 levels of nano-silver particles, including 0, 20, 40, 60, 80 and 100 mg.kg-1 and 6 levels of salinity 0, 30, 60, 90, 120, 150, 180 mmol.l-1. Salinity treatments were prepared using NaCl and CaCl2 at the rate of 1:1 in Hogland solution of 50%. The germination percentages, germination speed, vigor index, were also determined. Results showed that the application of nano-silver particles at the concentration of 40 mg.kg-1 led to the increases in germination percentage and improved the resistance to salinity conditions. the application of nano silver at suitable levels during germination process may enhance germination traits, plant growth and resistance to salinity conditions in basil seedlings.