Effect of Cultivar, Growth Regulators and Light During Incubation on Induction of Haploid in Lisianthus (Eustoma grandiflorum) through Microspore Culture

Lisianthus (Eustoma grandiflorum) is an almost new flower that Categorized between ten top cut flowers in the world, recently. In the persent study, the microspore culture of lisianthus flowers were conducted for the first time in the world. Also, the rate androgenesis in microspore culture in seven lisianthus cultivars were compared in the same growth conditions. Moreover, in a separate experiment, the influence of growth regulators on induction of haploids in lisianthus via microspore culture were studied. The interactions of 2,4-D (0/5, 1 and 2 mg/l) and kinetin (0/1, 0/5 and 1 mg/l) were compared; Also, the interaction of NAA (0/5 and 1 mg/l) and kinetin (0/5 and 1 mg/l) in a factorial experiment in a completely randomized design with 3 × 2 and 2 × 2 factors, each with three replicates were studied. Results revealed that not only all cultivars showed the positive response to androgenesis; but also, most of changes were happened in two cultivars named ‘Mariachi Pure White’ and ‘Mariachi Blue Picotee Improved’. Suspensor were formed in 0/5 mg/l NAA with 1 mg/l kineten. For 2,4-D (0/5 mg/l) and kineten (0/1 and 0/5 mg/l) induction of cell division and formation of multicellular structures were seen, Also, nuclear divisions were induced by 2 mg/l 2,4-D with 1 mg/l kinetin. In other experiment, the effect of light was studied during incubation with 16 hours lighting and 8 hours darkness. Results showed that white light did not expose any significant effect on isolated microspores.