SSR Polymorphism in Sterile, Low Fertile and Fertile of Different Citrus species

Simple sequence repeats (SSR) polymorphism in citrus including male sterile, sterile, low fertile and fertile was examined by analyzing 28 representative accessions sampled from north of Iran. Fifty four polymorphic alleles with an average of 4.2 alleles per primer were detected from eight pairs of used primers. The lowest number of alleles was observed in TAA27, CTT01, CCSM18 and ATC09 loci with only three alleles and the highest number of alleles was observed in TAA15 locus with eight alleles. Polymorphic information content (PIC) values changed from 0.34 (AG14) to 0.90 (CCSM18). Genetic similarities among accessions were calculated according to Jaccard Similarity Index and used to construct a dendrogram based on the unweighted pair group method using arithmetic averages (UPGMA) which put the 28 samples into four major groups (A, B, C and D). The cultivars of male sterile Satsuma mandarin were clustered into group A; those of orange, grapefruit and Page (a complex hybrid) into group B; mandarin cultivars into group C; and lemon Lisbon into group D.