Anti-oxidant and anti-microbial activities of two types of honey by change in bees, diet in comparison with other honey productis in Abestan region of Khorramabad province

Honey is a natural product of plant secretions collected by bees then after some changes are saved in hive. Honey is rich in enzymatic and non-enzymatic anti-oxidants such as Catalase, ascorbic acid, flavonoids and alkaloids. The important feature of honey is also antimicrobial activity. Also entering some ingredients into bees, diet can increase of effectiveness the nutritional value of honey So the anti-oxidant and anti-microbialactivities of two types of honey by change in bees,diet incomparison with the other honey of the region were studied. Three different types of diet given to bees including apple juice, flowers and root extract of Licorice and region flowers so that three types of honey were produced. The honey affected on standard bacteria and Candida albicans with several antibiotics to evaluate of antimicrobial activity according to agar well diffusion assay. The amount of antioxidant compounds, measured according total phenol test. For measurement of antioxidant effects of honey, three tests of FRAP (ferric reducing antioxidant power), DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABT (3-ethylbenzothiazoline-6-sulphonic acid) were used. In this experiment, Licorice honey had highest antimicrobial activity especially against pseudomonas aeruginosa. The tested honey had a significant difference in total amount of phenol. In all three methods (FRAP, DPPH and ABT), apple honey had the highest antioxidant activity. According to the results of this study, entering some effective and natural materials into bees’ diet can cause a revolution in the honey product industry, as a drug, and health of society.