Development of a Double Glass Mounting Method Using Formaldehyde Alcohol Azocarmine Lactophenol (FAAL) and its Evaluation for Permanent Mounting of Small Nema¬todes

: Permanent slide preparation of nematodes especially small ones is time consuming, difficult and they become scarious margins. Regarding this prob­lem, a modified double glass mounting method was developed and com­pared with classic method. A total of 209 nematode samples from human and animal origin were fixed and stained with Formaldehyde Alcohol Azocarmine Lactophenol (FAAL) followed by double glass mounting and classic dehydration method us­ing Canada balsam as their mounting media. The slides were evaluated in differ­ent dates and times, more than four years. Different photos were made with differ­ent magnification during the evaluation time. The double glass mounting method was stable during this time and compa­rable with classic method. There were no changes in morphologic struc­tures of nematodes using double glass mounting method with well-defined and clear differentiation between different organs of nematodes in this method. Using this method is cost effective and fast for mounting of small nematodes comparing to classic method.