Evaluating the effectiveness of Native Trichoderma Species in Production of Extracellular Enzymes During Interaction with Plant Pathogenic Fungus Fusarium oxysporum

Trichoderma species are of important antagonist agents in plant pathogens biocontrol. These agents utilize different mechanisms in their biocontrol activities such as production of extracellular enzymes. In order to evaluate the ability of antagonist and pathogenic fungus in producing extracellular enzymes, specific culture media such as amylase, cellulose, laccase, lipase and protease were prepared. Subsequently, each antagonist and pathogenic fungus was separately inoculated on the appropriate media and incubated for 3-5 days in 25±1°C. Results indicated that T. atroviridae (1-3), T. virens (6011) and F. oxysporum produced amylase, cellulase and laccase. T. koningii was only able to produce lipase enzyme. T. harzianum (Ahmedabad) produced amylase and cellulase but not laccase. T. atroviredae (6022) produced laccase but, was unable to produce amylase and cellulase enzymes. The results of the dual cultures, leads to T. harzianum (Ahmedabad) compared with other Trichoderma species had maximum growth inhibition on amylase and cellulase medium. While the dual culture assay of laccase medium indicated that the higher activity percentage of growth inhibition in F. oxysporum species compared with antagonists was significantly different.However, it seems each substrate during interaction of Trichoderma species with Fusarium sp., had different specific action due to enzyme production.