In ovo injection of nano silver, thyme and savory extracts to broiler breeders eggs and their effect on post-hatch immunological parameters

This experiment was conducted to examine the effects of in ovo injection of Nano silver, Thyme and Savory extracts to broiler breeder’s eggs and their effect on immunologic parameters. A total of 495 fertilized eggs were used in the pre-Experiment with 11 treatments by both 3 replications and 15 eggs in each. Treatments including: two controls (1- with injection and 2- without injection), treatment 3-11 injected with: 30, 45 and 60 ppm of Nano silver, 100, 150, 175 ppm of Thyme and Savory extracts. The eggs injected at 17th day of incubation. Result indicated that IgA and IgM were affected by treatment so that the IgA levels were significantly higher in all of the treatment rather than the control and the IgM levels were significant higher in 175 ppm thyme extract in comparison to 60 ppm Nano silver. Also in 14 and 21 days, response of the skin to Di-nitrocholorobanzen (DNCB), bursa and spleen of weights were not significant. The results showed that IgM and IgA, had better response to injection of Thyme and Savory extracts than Nano silver.