Evaluation of Resistance to Stripe Rust in Doubled Haploid Lines of Bread Wheat

In the present study, virulence factors of two isolates of stripe rust, collected from Karaj and Kermanshah, Iran were determined on differential lines, and for genes Yr2, 2+, 6, 6+, 7, 7+, 8, 9, 17, 18, 25, 26, 27, 32, A, DN virulence was observed in both isolates. Reaction of 150 doubled haploid lines together with parents and check cultivars to stripe rust (Puccinia striiformis f.sp. tritici) was evaluated at seedling and adult plant stages by assessment of coefficient of infection and infection type in an agmented design. The results of analysis of variance showed significant differences among check cultivars for infection type and coefficient of infection of two races of Karaj (7E158A+, Yr27) and Kermanshah (110E158A+, Yr27). Using Ward’s method of cluster analysis based on infection type and coefficient of infection of both races, doubled haploid lines and 7E158A+,Yr27 were classified in two groups of susceptible and resistant. Overall, 28 doubled haploid lines at both stages of seedling and adult plant showed resistance reaction to race of Karaj which indicates the probability of carrying one or more minor genes of adult plant resistance in addition to seedling major genes.