Comparison of Amount of Callus Induction in Juniperus Excelsa on MS and WPM Culture Media Using Different Concentration of NAA, 2,4-D and Kin

Juniper (Juniperus excelsa L.), a plant with very slow growth and belong to Cuperssaceae family, has medicinal characteristics and is rich in aromatic essential oils and valuable in Pharmaceutical and perfumery industries. Therefore, to use tissue culture an experiment was undertaken using lateral buds explants. Present study has been fulfilled using factorial experiments based on completely randomized designs with 3 replications using MS (Murashige and Skoog) and WPM (Woody Plants Medium) in two separate experiments that supplemented with different concentrations of Kin (0, 0.1, 0.2 mgl-1) with 2,4-D (0, 1, 2, 3, 4 mgl-1) and NAA (0, 1, 2, 3, 4 mgl-1) with Kin (0, 0.1, 0.2 mgl-1). The highest average weight of calli was produced on WPM medium containing 3 mgl-1 NAA in combination with 0.2 mgl-1 Kin. The highest diameter of calli was formed on WPM medium containing 4 mgl-1 NAA in combination with 0.1 mgl-1 Kin and the highest percentage of callus induction was also obtained on WPM medium containing 3 mgl-1 2,4-D in combination with 0.2 mgl-1 Kin.