Effect of different cytokinins on micropropagation of Lavandula vera

The effect of different cytokinins on micropropagation of lavandula vera were investigated. Two nodal explants from greenhouse plant were cultured in MS medium supplemented with benzyl aminopurine (BAP), 2-isopentenyl adenine (2ip), Kinetin (Kin) solely or in combination. Results indicated that shoot proliferation depended on type and concentration of hormone. The highest number of nods and new shoots per explants and stem length was produced in the medium with BAP(2 mgl-1). Growth of internodes was significantly increased in the medium with Kin and 2ip (1 mgl-1).In the subculture media, the highest number of nodes and new shoots per explants was obtained in the medium with BAP (1 mgl-1). Maximum of internode length was achieved in the medium with Kin (2 mgl-1). In vitro shoots were rooted in vitro or ex vitro. By using in vitro method, microshoots were cultured in the media supplemented with different concentration of indole butyric acid (IBA) and Kin. In ex vitro method, the base of microshoots was treated with IBA (300 mgl-1). The highest number of roots as well as length of roots was obtained in ex vitro rooting method.