Optimization of T DNA transformation and transgenic plant production of Datura metel L

In this study conditions for transformation of Datura metel was optimized. T-DNA from pZM1047 containing GUS and NPTII genes was transferred to Datura plant. Bacterial suspension was used for co culture with leaf segments., the they were transferred to MS medium supplemented with 1 mg/L BAP as regenerated medium. After 15-20 days calli with regeneration marks was appeared. The whole regenerated transgenic plants on kanamycin were checked using PCR. Transformation rate was about 13% which is 2% higher than previous study reported by others. In this study conditions for transformation of Datura metel was optimized. T-DNA from pZM1047 containing GUS and NPTII genes was transferred to Datura plant. Bacterial suspension was used for co culture with leaf segments., the they were transferred to MS medium supplemented with 1 mg/L BAP as regenerated medium. After 15-20 days calli with regeneration marks was appeared. The whole regenerated transgenic plants on kanamycin were checked using PCR. Transformation rate was about 13% which is 2% higher than previous study reported by others.