Effects of aqueous and ethanolic extract of Rosa damascena mill L. against human gastric Cancer cells

Gastric cancer is one of the important morbidity factors in the world. There are many unique plant species that should be explored for anti-cancer components. Goal: In this project, anti-cancer effects of aqueous and ethanolic extract of Persia rose (Rosa damascena mill L.) against human gastric cancer cell line (AGS) was studied. Experimental procedure: Eight different concentrations of extracts as well as 5-fluorouracil was applied on the cells. The toxicity of the extracts, their inhibitory effects on proliferation and apoptotic effects were studied by MTT, BrdU and TUNEL assays respectively. The results showed that all concentrations of aqueous and ethanolic extracts reduced viability of the AGS cells significantly. The IC50 of these extracts on AGS cells was determined 2.517 and 3.887 µg/ml respectively.BrdU assay also showed the inhibitory effect of extracts on proliferation of AGS cells (in comparison to fibroblast cells). The proliferation of AGS cells was decreased as the concentration of both of the extracts was increased. Also, the toxicity and anti-proliferative effect of ethanolic extract was more than aqueous extract. The rate of apoptosis was determined 90% for both aqueous and ethanolic extract. Aqueous and ethanolic extract of Rosa damascena mill L. reduced viability and proliferation and induce apoptosis in human gastric cancer cells through different pathways.